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Microscopes


*** LEICA SP8 Lightning DLS - basic specifications:

heimo.wolinski(at)uni-graz.at

  • Leica DMi8 CS DLS inverse research microscope with spectral detection
  • Light-sheet module
  • High-content screening platform

Fluorescence unit:

  • Sola-SMII LED fluorescence illumination
  • Filter cubes (excitation – emission):
    BP 350/50 - BP 460/50 (DAPI)
    BP 480/40 - BP 527/30 (FITC)
    BP 546/10 - BP 585/40 (Rhodamin)
  • DFC9000 GTC DLS sCMOS camera

Detectors/stages/software:

  • Super Z galvo scanner stage (1.5 mm z-distance)
  • Automated stage controller (mosaic scanning, positioning scans, microtiter plate scanning, multi-dimensional large-field overview scans; multiplex imaging)
  • 8kHz resonant scanner (ultrafast scanning)
  • 2 photomultiplier tubes
  • 3 noise-less hybrid detectors (HyDs)
  • LAS X Lightning Expert package ("superresolution").
  • FRET, FRAP, FLIP, Ca2+ imaging wizards

Laser lines:

  • 405 nm DMOD for e.g. DAPI excitation
  • 458 nm, 476 nm, 488 nm, 496 nm, 514 nm argon laser
  • 561 nm DPSS laser
  • 633 nm HeNe laser

Objectives - confocal unit:

  • HC FLUOTAR L 16x/0.6 IMM CORR VISIR
  • HC PL APO 20x/0.75 IMM CORR CS2
  • HC FLUOTAR L 25x/0.95 W VISIR 0.17
  • HC PL APO 63x/1.40 OIL CS2
  • HC PL APO 63x/1.30 GLYC CORR CS2

Illumination objectives - light-sheet module:

  • L 1.6x/0.05 DLS
  • HC PL FLUOTAR 2.5x/0.07
  • HC PL FLUOTAR 4x/0.13
  • HC PL FLUOTAR 5x/0.15

Detection objectives - light-sheet module:

  • HC FLUOTAR L25x/0.95 W DLS
  • HC APO L10x/0.30 W DLS
  • HC PL FLUOTAR 5x/0.15 IMM DLS

Sample rotation device for light-sheet module


*** LEICA TCS SP5 AOBS CARS SHG 2-photon
heimo.wolinski(at)uni-graz.at

  • Leica SP 5 confocal laser scanning microscope
  • Hybrid photon detectors capable of photon counting
  • Objectives: 100x 1.4 oil, 63x 1.4 oil, 63x 1.25 water, 63x 1.3 glycerol, 40x 1.25 oil, 40x 1.2 air, 25x 0.95 W IR, 10x.
  • Differential interference contrast (DIC) optics.
  • Laser lines: Argon laser: 458nm, 476nm 488nm/, 496nm/, 514nm/; DPSS-laser: 561nm; HeNe-laser: 633nm.
  • Resonant scanner: 8000 Hz (25frames/sec 512x512).
     
  • On stage incubation system (CO2, temperature, humidity control).
  • Mini perfusion chamber (BiopTechs).
     
  • Coherent anti-Stokes Raman Scattering (CARS) system
     
  • Second Harmonic Generation (SHG) system
     
  • 2-photon system

    based on PicoEMERALD laser light source (APE GmbH, Germany).
     
  • Standard non-descanned detectors
  • NDD high-end hybrid detector

 

*** NIKON TIRF N-STORM inverted superresolution microscope
heimo.wolinski(at)uni-graz.at

 

*** Further microscopes

 

External resources 

Specialized confocal laser scanning microscope with movable objective and electrophysiology setup. Institute of Zoology.
boris.chagnaud(at)uni-graz.at

Seniors Scientist, Ph.D.

Heimo Wolinski

Humboldtstrasse 50/II, 8010 Graz

Phone:+43 (0)316 380 - 5489


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