Mikroskope

*** LEICA SP8 Lightning DLS - basic specifications:
heimo.wolinski(at)uni-graz.at

• Leica DMi8 CS DLS inverse research microscope with spectral detection
• Light-sheet module
• High-content screening platform

Fluorescence unit:

• Sola-SMII LED fluorescence illumination
• Filter cubes (excitation – emission):
  BP 350/50 - BP 460/50 (DAPI)
  BP 480/40 - BP 527/30 (FITC)
  BP 546/10 - BP 585/40 (Rhodamin)
• DFC9000 GTC DLS sCMOS camera

Detectors/stages/software:

• Super Z galvo scanner stage (1.5 mm z-distance)
• Automated stage controller (mosaic scanning, positioning scans, microtiter plate scanning, multi-dimensional large-field overview scans; multiplex imaging)
• 8kHz resonant scanner (ultrafast scanning)
• 2 photomultiplier tubes
• 3 noise-less hybrid detectors (HyDs)
• LAS X Lightning Expert package ("superresolution").
• FRET, FRAP, FLIP, Ca2+ imaging wizards

Laser lines:

• 405 nm DMOD for e.g. DAPI excitation
• 458 nm, 476 nm, 488 nm, 496 nm, 514 nm argon laser
• 561 nm DPSS laser
• 633 nm HeNe laser

Objectives - confocal unit:

• HC FLUOTAR L 16x/0.6 IMM CORR VISIR
• HC PL APO 20x/0.75 IMM CORR CS2
• HC FLUOTAR L 25x/0.95 W VISIR 0.17
• HC PL APO 63x/1.40 OIL CS2
• HC PL APO 63x/1.30 GLYC CORR CS2

Illumination objectives - light-sheet module:

• L 1.6x/0.05 DLS
• HC PL FLUOTAR 2.5x/0.07
• HC PL FLUOTAR 4x/0.13
• HC PL FLUOTAR 5x/0.15

Detection objectives - light-sheet module:

• HC FLUOTAR L25x/0.95 W DLS
• HC APO L10x/0.30 W DLS
• HC PL FLUOTAR 5x/0.15 IMM DLS

Sample rotation device for light-sheet module

*** LEICA TCS SP5 AOBS CARS SHG 2-photon
heimo.wolinski(at)uni-graz.at

• Leica SP 5 confocal laser scanning microscope
• Hybrid photon detectors capable of photon counting
• Objectives: 100x 1.4 oil, 63x 1.4 oil, 63x 1.25 water, 63x 1.3 glycerol, 40x 1.25 oil, 40x 1.2 air, 25x 0.95 W IR, 10x.
• Differential interference contrast (DIC) optics.
• Laser lines: Argon laser: 458nm, 476nm 488nm/, 496nm/, 514nm/; DPSS-laser: 561nm; HeNe-laser: 633nm.
• Resonant scanner: 8000 Hz (25frames/sec 512x512).
   
• On stage incubation system (CO2, temperature, humidity control).
• Mini perfusion chamber (BiopTechs).
   
• Coherent anti-Stokes Raman Scattering (CARS) system
   
• Second Harmonic Generation (SHG) system
   
• 2-photon system
  
  based on PicoEMERALD laser light source (APE GmbH, Germany).
   
• Standard non-descanned detectors
• NDD high-end hybrid detector

*** NIKON TIRF N-STORM inverted superresolution microscope
heimo.wolinski(at)uni-graz.at

*** Weitere Geräte

• LEICA M165 FC Stereo Fluorescence microscope system 
  ronald.kühnlein@uni-graz.at
   
• NIKON Eclipse Ti-E inverted fluorescence microscope 
  stefan.schild(at)uni-graz.at
   
• Nikon Eclipse TE300 inverted fluorescence microscope; phase contrast, differential interference contrast, long-working distance objectives, automatic z-stepping, heating chamber; mercury lamp. 
  stefan.schild(at)uni-graz.at
   
• Leica DM 6000 B inverted fluorescence microscope; phase contrast; DIC; heating stage
  dagmar.zweytick(at)uni-graz.at
   
• Leica DM6 B upright automated fluorescence microscope with LED illumination
  helmut.bergler(at)uni-graz.at
   

External resources 

Specialized confocal laser scanning microscope with movable objective and electrophysiology setup. Institute of Zoology.
boris.chagnaud(at)uni-graz.at